Yeasen Molefuture Biotechnology Unveils Breakthrough in Enzyme Engineering: Enhanced Bst DNA Polymerase Published in ACS Catalysis

Yeasen Molefuture Biotechnology is thrilled to share our groundbreaking research published in ACS Catalysis (Impact Factor 11.3), showcasing a major leap in enzyme engineering. By harnessing Fluorescence-Activated Droplet Sorting (FADS) technology, we’ve developed a highly thermostable Bst DNA Polymerase that transforms Loop-mediated Isothermal Amplification (LAMP) assays.

Our innovation overcomes critical limitations of wild-type Bst DNA Polymerase, including slow reaction speeds, limited thermostability, false positives in LAMP, and poor reagent stability. The engineered mutants (M2 and M3) deliver remarkable improvements:

• Speed: Reduced LAMP detection time from 40 to 10 minutes.

• Thermostability: Achieved a T151/2 of 70.2°C, enabling High-Temperature LAMP (HT-LAMP) at 70°C to eliminate false positives.

• Stability: Ensured lyophilized reagents remain stable for over 6 months at 50°C.

This cutting-edge enzyme is now available as Hieff® Super Bst DNA Polymerase (Cat#14410), ready to power advanced diagnostics and research.

• Research Article: https://doi.org/10.1021/acscatal.4c07614

• Product Page: https://www.yeasen.com/products/detail/12842

Interested in experiencing this innovation? Contact us to request a trial pack of Hieff® Super Bst DNA Polymerase (2,000 U/μL).