The ZymeEditor platform seamlessly integrates directed evolution and rational design techniques. It autonomously incorporates cutting-edge enzyme evolution technologies, including Fluorescence-Activated Droplet Sorting (FADS), alongside traditional methods like Microtiter Plate Screening (MTPS) and Computer-Aided Rational Design (CARD). Through cross-screening utilizing these technologies, ZymeEditor significantly enhances the efficacy of enzyme evolution, leading to the optimization of various enzyme properties such as activity, stability, selectivity, and more.
FADS platform
丨 1）Directed Evolution Technology
This technology combines molecular probe technology with high-throughput single-cell sorting, enabling rapid enrichment of beneficial mutation sites. It shortens the evolution cycle, enhances the screening success rate, and significantly reduces screening costs.
Conduct multi-scale bioinformatics analysis targeted at evolutionary objectives to identify key amino acid residues.
Utilize optimized strategies and comprehensive computer-aided modules to achieve efficient design of mutants.
Leverage accumulated extensive physicochemical data on mutants and establishing machine learning models based on structure-function relationships.
Precisely and efficiently generate beneficial mutants based on specific targets.
丨 2）Rational Design Technology
Based on specific evolutionary needs, by analyzing the relationship between structure and function, we rationally design accurate and reliable mutation sites to improve the accuracy and speed of protein modification.
Conduct multi-scale bioinformatics analysis for evolutionary targets to obtain key amino acid sites
Use optimized transformation ideas and complete computer-aided modules to achieve efficient design of mutants
Establish a machine learning model with the help of accumulated massive physical and chemical data of mutants and structure-activity relationships
Accurately and efficiently output beneficial mutants according to protein modification targets
